A novel rat robot controlled by electrical stimulation of the nigrostriatal pathway.

OBJECTIVE: Artificial manipulation of animal movement could offer interesting advantages and potential applications using the animal's inherited superior sensation and mobility. Although several behavior control models have been introduced, they generally epitomize virtual reward-based training models. In this model, rats are trained multiple times so they can recall the relationship between cues and rewards. It is well known that activation of one side of the nigrostriatal pathway (NSP) in the rat induces immediate turning toward the contralateral side. However, this NSP stimulation-induced directional movement has not been used for the purpose of animal-robot navigation. In this study, the authors aimed to electrically stimulate the NSP of conscious rats to build a command-prompt rat robot. METHODS: Repetitive NSP stimulation at 1-second intervals was applied via implanted electrodes to induce immediate contraversive turning movements in 7 rats in open field tests in the absence of any sensory cues or rewards. The rats were manipulated to navigate from the start arm to a target zone in either the left or right arm of a T-maze. A leftward trial was followed by a rightward trial, and each rat completed a total of 10 trials. In the control group, 7 rats were tested in the same way without NSP stimulation. The time taken to navigate the maze was compared between experimental and control groups. RESULTS: All rats in the experimental group successfully reached the target area for all 70 trials in a short period of time with a short interstimulus interval (< 0.7 seconds), but only 41% of rats in the control group reached the target area and required a longer period of time to do so. The experimental group made correct directional turning movements at the intersection zone of the T-maze, taking significantly less time than the control group. No significant difference in navigation duration for the forward movements on the start and goal arms was observed between the two groups. However, the experimental group showed quick and accurate movement at the intersection zone, which made the difference in the success rate and elapsed time of tasks. CONCLUSIONS: The results of this study clearly indicate that a rat-robot model based on NSP stimulation can be a practical alternative to previously reported models controlled by virtual sensory cues and rewards.

Fabrication of Convex PDMS-Parylene Microstructures for Conformal Contact of Planar Micro-Electrode Array.

Polymer-based micro-electrode arrays (MEAs) are gaining attention as an essential technology to understand brain connectivity and function in the field of neuroscience. However, polymer based MEAs may have several challenges such as difficulty in performing the etching process, difficulty of micro-pattern generation through the photolithography process, weak metal adhesion due to low surface energy, and air pocket entrapment over the electrode site. In order to compensate for the challenges, this paper proposes a novel MEA fabrication process that is performed sequentially with (1) silicon mold preparation; (2) PDMS replica molding, and (3) metal patterning and parylene insulation. The MEA fabricated through this process possesses four arms with electrode sites on the convex microstructures protruding about 20 µm from the outermost layer surface. The validity of the convex microstructure implementation is demonstrated through theoretical background. The electrochemical impedance magnitude is 204.4 ± 68.1 kΩ at 1 kHz. The feasibility of the MEA with convex microstructures was confirmed by identifying the oscillation in the beta frequency band (13-30 Hz) in the electrocorticography signal of a rat olfactory bulb during respiration. These results suggest that the MEA with convex microstructures is promising for applying to various neural recording and stimulation studies.

Cross detection for odor of metabolic waste between breast and colorectal cancer using canine olfaction.

Although several studies have been performed to detect cancer using canine olfaction, none have investigated whether canine olfaction trained to the specific odor of one cancer is able to detect odor related to other unfamiliar cancers. To resolve this issue, we employed breast and colorectal cancer in vitro, and investigated whether trained dogs to odor related to metabolic waste from breast cancer are able to detect it from colorectal cancer, and vice versa. The culture liquid samples used in the cultivation of cancerous cells (4T1 and CT26) were employed as an experimental group. Two different breeds of dogs were trained for the different cancer odor each other. The dogs were then tested using a double-blind method and cross-test to determine whether they could correctly detect the experimental group, which contains the specific odor for metabolic waste of familiar or unfamiliar cancer. For two cancers, both dogs regardless of whether training or non-training showed that accuracy was over 90%, and sensitivity and specificity were over 0.9, respectively. Through these results, it was verified that the superior olfactory ability of dogs can discriminate odor for metabolic waste of cancer cells from it of benign cells, and that the specific odor for metabolic waste of breast cancer has not significant differences to it of colorectal cancer. That is, it testifies that metabolic waste between breast and colorectal cancer have the common specific odor in vitro. Accordingly, a trained dogs for detecting odor for metabolic waste of breast cancer can perceive it of colorectal cancer, and vice versa. In order to the future work, we will plan in vivo experiment for the two cancers and suggest research as to what kind of cancers have the common specific odor. Furthermore, the relationship between breast and colorectal cancer should be investigated using other research methods.

Publisher Correction: Manipulation of Rat Movement via Nigrostriatal Stimulation Controlled by Human Visually Evoked Potentials.

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.

Manipulation of Rat Movement via Nigrostriatal Stimulation Controlled by Human Visually Evoked Potentials.

Here, we report that the development of a brain-to-brain interface (BBI) system that enables a human user to manipulate rat movement without any previous training. In our model, the remotely-guided rats (known as ratbots) successfully navigated a T-maze via contralateral turning behaviour induced by electrical stimulation of the nigrostriatal (NS) pathway by a brain- computer interface (BCI) based on the human controller's steady-state visually evoked potentials (SSVEPs). The system allowed human participants to manipulate rat movement with an average success rate of 82.2% and at an average rat speed of approximately 1.9 m/min. The ratbots had no directional preference, showing average success rates of 81.1% and 83.3% for the left- and right-turning task, respectively. This is the first study to demonstrate the use of NS stimulation for developing a highly stable ratbot that does not require previous training, and is the first instance of a training-free BBI for rat navigation. The results of this study will facilitate the development of borderless communication between human and untrained animals, which could not only improve the understanding of animals in humans, but also allow untrained animals to more effectively provide humans with information obtained with their superior perception.

Decoding intravesical pressure from local field potentials in rat lumbosacral spinal cord.

Chronic monitoring of intravesical pressure is required to detect the onset of intravesical hypertension and the progression of a more severe condition. Recent reports demonstrate the bladder state can be monitored from the spiking activity of the dorsal root ganglia or lumbosacral spinal cord. However, one of the most serious challenges for these methods is the difficulty of sustained spike signal acquisition due to the high-electrode-location-sensitivity of spikes or neuro-degeneration. Alternatively, it has been demonstrated that local field potential recordings are less affected by encapsulation reactions or electrode location changes. Here, we hypothesized that local field potential (LFP) from the lumbosacral dorsal horn may provide information concerning the intravesical pressure. LFP and spike activities were simultaneously recorded from the lumbosacral spinal cord of anesthetized rats during bladder filling. The results show that the LFP activities carry significant information about intravesical pressure along with spiking activities. Importantly, the intravesical pressure is decoded from the power in high-frequency bands (83.9-256 Hz) with a substantial performance similar to that of the spike train decoding. These findings demonstrate that high-frequency LFP activity can be an alternative intravesical pressure monitoring signal, which could lead to a proper closed loop system for urinary control.

Propagation of spoof surface plasmon on metallic square lattice: bending and splitting of self-collimated beams.

The propagation characteristics of spoof surface plasmon modes are studied in both real and reciprocal spaces. From the metallic square lattice, we obtain constant frequency contours by directly measuring electric fields in the microwave frequency regime. The anisotropy of the measured constant frequency contour supports the presence of the negative refraction and the self-collimation which are confirmed from measured electric fields. Additionally, we demonstrate the spoof surface plasmon beam splitter in which the splitting ratio of the self-collimated beam is controlled by varying the height of rods.

Resonant transmission of self-collimated beams through coupled zigzag-box resonators: slow self-collimated beams in a photonic crystal.

The resonant transmission of self-collimated beams through zigzag-box resonators is demonstrated experimentally and numerically. Numerical simulations show that the flat-wavefront and the width of the beam are well maintained after passing through zigzag-box resonators because the up and the down zigzag-sides prevent the beam from spreading out and the wavefront is perfectly reconstructed by the output zigzag-side of the resonator. Measured split resonant frequencies of two- and three-coupled zigzag-box resonators are well agreed with those predicted by a tight binding model to consider optical coupling between the nearest resonators. Slowing down the speed of self-collimated beams is also demonstrated by using a twelve-coupled zigzag-box resonator in simulations. Our work could be useful in implementing devices to manipulate self-collimated beams in time domain.

High-speed measurement of three-dimensional surface profiles up to 10 µm using two-wavelength phase-shifting interferometry utilizing an injection locking technique.

High-speed two-wavelength phase-shifting interferometry is presented. The technique is aimed at high-speed in-line inspection of spacers in liquid crystal display panels or wafer bumps where the measuring range is well determined and high-speed measurements are essential. With our test setup, the measuring range is extended to 10 µm by using two injection locked frequency scanning lasers that offer fast and equidistant phase shifting of interference fringes. A technique to determine the unwrapped phase map in a frequency scanning phase-shifting interferometry without the ordinary phase-unwrapping process is proposed.

Ring-type Fabry-Pérot filter based on the self-collimation effect in a 2D photonic crystal.

We propose a ring-type Fabry-Pérot filter (RFPF) based on the self-collimation effect in photonic crystals. The transmission characteristics of self-collimated beams are experimentally measured in this structure and compared with the results obtained with the simulations. Bending and splitting mechanisms of light beams by the line defects introduced into the RFPF are used to control the self-collimated beam. Antireflection structures are also employed at the input and output photonic crystal interfaces in order to minimize the coupling loss. Reflectance of the line-defect beam splitters can be controlled by adjusting the radius of defect rods. As the reflectance of the line-defect beam splitters increases, the transmission peaks become sharper and the filter provides a Q-factor as high as 1037. Proposed RFPF can be used as a sharply tuned optical filter or as a spectrum analyzer based on the self-collimation phenomena of photonic crystals. Furthermore, it is suitable for a building block of photonic integrated circuits, as it does not back reflect any of the incoming self-collimated beams owing to the antireflection structure applied.

Asymmetric Mach-Zehnder filter based on self-collimation phenomenon in two-dimensional photonic crystals.

A two-dimensional photonic crystal asymmetric Mach-Zehnder filter (AMZF) based on the self-collimation effect is studied by numerical simulations and experimental measurements in microwave region. A self-collimated beam is effectively controlled by employing line-defect beam splitters and mirrors. The measured transmission spectra at the two output ports of the AMZF sinusoidally oscillate with the phase difference of pi in the self-collimation frequency range. Position of the transmission peaks and dips can be controlled by varying the size of the defect rod of perfect mirrors, and therefore this AMZF can be used as a tunable power filter.

Silver superlens using antisymmetric surface plasmon modes.

Silver lenses having super-resolution are analyzed in terms of antisymmetric modes of surface plasmon which have the ability to amplify evanescent waves in UV region. Antisymmetric surface plasmon modes excited by subwavelength grating enhances the resolution and contrast of silver superlens. By using a 20 nm-thick silver superlens, the half-pitch resolution of approximately lambda(0)/8 can be achieved with good contrast at a free space wavelength of 435 nm. The resolution of silver superlens can also be improved using shorter illumination wavelength. We show that the thinner the lens, the better the imaging ability of the silver superlens due to the excitation of antisymmetric surface plasmon modes of higher propagation wave vectors. The thickness of lens is varied from 20 to 40 nm in a three layer system, SiO(2)-Ag-SiO(2). Obtained results illustrate that practical application for patterning periodic structures with good contrast and penetration depth can be achieved by using antisymmetric surface plasmon modes.

Surface plasmon polariton resonance and transmission enhancement of light through subwavelength slit arrays in metallic films.

In this study, we present experimentally measured transmission enhancement of microwaves through periodic slit arrays in metallic films. Enhanced transmission peaks and sharp transmission dips are clearly observed around the theoretically expected surface plasmon polariton(SPP) resonance frequencies. Dependence of the transmittance spectra on the geometrical properties of slits is also demonstrated by varying the slit width, slit periodicity and the thickness of metallic films. Transmission peaks and dips are originated from the coupling between the incident light and SPPs which are caused by the slit array that acts like a grating coupler. The obtained results are theoretically explained by solving the Maxwell's equations and by the diffraction theory with appropriate boundary conditions, and they are in good agreement with those calculated by the finite-difference time-domain method.

High speed phase shifting interferometry using injection locking of the laser frequency to the resonant modes of a confocal Fabry-Perot cavity.

We present a high speed phase shifting interferometer which utilizes the self injection locking of a frequency tunable laser diode. By using a confocal Fabry-Perot cavity made of ultra low expansion glass, and linearly modulating the laser diode current, the laser frequency could be injection locked to the resonant modes of the Fabry-Perot cavity consecutively. It provided equal phase steps to the interferograms which are ideal to be analyzed by the Carré algorithm. The phase step error was evaluated to be about 3 MHz which corresponds to 0.2 nm in length measurement. With this technique, profile measurements are insensitive to external vibration since four 640x480 pixels images can be acquired within 4 ms. Difference of two profile measurements, each made with and without vibration isolation, respectively, was evaluated to be 0.5 nm (rms).

Efficient beaming of self-collimated light from photonic crystals.

We propose a novel structure for achieving highly efficient beaming of self-collimated light from two-dimensional photonic crystals. The finite-difference time-domain simulations show that both enhanced transmission and highly directional emission of self-collimated beams from photonic crystals are achieved by using the bending and splitting of self-collimated beams in photonic crystals, and also by introducing an antireflection coating-like photonic crystal collimator to the exit surface of the structure. This structure is potentially important for highly efficient coupling of self-collimated beams from photonic crystals into conventional optical fibers and photonic crystal waveguides.

Reflection minimization at two-dimensional photonic crystal interfaces.

We propose a method to design antireflection structures to minimize the reflection of light beams at the interfaces between a two-dimensional photonic crystal and a homogeneous dielectric. The design parameters of the optimal structure to give zero reflection can be obtained from the one-dimensional antireflection coating theory and the finite-difference time-domain simulations. We examine the performance of a Mach-Zehnder interferometer utilizing the self-collimated beams in two-dimensional photonic crystals with and without the optimal antireflection structure introduced. It is shown that the optimal antireflection structure significantly improves the performance of the device.

Dispersion and birefringence of irregularly microstructured fiber with an elliptic core.

We have investigated the dispersion and birefringence of an irregularly microstructured fiber with an elliptic silica core and irregular airholes. The polarization-dependent output power through the fiber reveals two well-defined principal-axis modes despite the irregularity of airholes. The dispersion of the fiber is measured in the range of 680 to 1000 nm using the Mach-Zehnder interferometric technique with sub-10 fs laser pulses, which yield two zero dispersion wavelengths at 683 and 740 nm for the two principal modes, respectively. The birefringence measured using the wavelength scanning method is about 0.0055 at 800 nm. It is also demonstrated that this irregularly microstructured fiber with high birefringence and short zero dispersion wavelength is useful for the one-octave-spanning supercontinuum generation suitable for an f-2f interferometric system.

Self-collimation phenomena of surface waves in structured perfect electric conductors and metal surfaces.

We demonstrate that surface waves in structured perfect electric conductor surfaces can be self-collimated using the finite-difference time-domain method. The self-collimation frequency is obtained from the equi-frequency contours of a perfect electric conductor patterned with an array of square holes. The field patterns of the self-collimated surface wave, obtained using the periodic boundary conditions, show that the surface waves propagate with almost no spreading. We also show that self-collimation phenomena can be observed for the hybrid surface plasmon waves in structured metal surfaces using the finite-difference time-domain method with the Drude model. It is shown that for a structured silver surface the self-collimation can be achieved at the frequencies in the infrared region.

Growth of the transient planar state in cholesteric liquid crystals.

Growth and propagation of the transient planar state in the transition of cholesteric liquid crystals from the homeotropic to the planar state was investigated by both experiments and numerical simulations for planar boundary conditions. Interference fringes observed in the measured reflection spectra during the evolution of the transient planar state have not been reported in the previous study. These interference fringes caused by a Fabry-Perot interferometric structure formed in the cell show that the transient planar state grows from the surfaces and propagates toward the bulk region in the planar sample. Our experimental results for the growth of transient planar state in planar boundary conditions are consistent with the previously reported simulation studies.